The advertised subproject will be carried out by a pre-doctoral researcher (“Early-stage Researcher” -ESR) at the European Laboratory for Non-Linear Spectroscopy (Lens) over a period of 12 months. The recruited researcher will work in the Biophotonics laboratory of LENS .

The novel ESR would work on the following objectives in cooperation with another ESR already enrolled and receive training in the following techniques:

- Study the tropisms of APIs in cleared CNS mouse tissues. Learn clearing techniques and light sheet microscopy.

- Study the binding affinity of a set of nanobodies against a new putative inhibitory domain of Nogo-

A in cells overexpressing a chimeric Nogo-A construct, and the mobility of the latter on the plasma

membrane. Learn cell imaging with advanced microscopy and single molecule tracking.

- Participate to the characterization of trafficking of nano/micro particles in cells and of diffusion rates in hydrogels in vitro

This subproject is fully funded by the Marie Skłodowska-Curie European Training Network „Bio2Brain“ (H2020-MSCA-ITN-2020). The monthly gross salary consists of a living allowance, mobility allowance and family allowance (if applicable). This is € 45,262,80 for 12 months which includes the single person’s mobility allowance, or €51,262.80 for 12 months, which includes the married person’s/dependents mobility allowance.

For more information on MSCA-ITN, please see: https://ec.europa.eu/research/mariecurieactions/.

The recruited researcher will have the opportunity to work as part of an international, interdisciplinary team of early stage researchers (ESRs), based at universities and industrial firms throughout Europe.

The successful candidate will attend a summer schools as well as an international meeting and will receive training on transferable skills. She/he is expected to disseminate the results through publications in peer-reviewed journals and presentations at international conferences.

Interested candidates are strongly advised, prior to applying, to read carefully the job description and to contact Martino Calamai at calamai@lens.unifi.it in case of any questions or doubts.

To apply, please send an email with the following documents to segreteria@lens.unifi.it: your complete CV, a half-page summary of master thesis, a letter of motivation (up to 1 page) which states your research experience and interests, and names and contact information of at least two academic referees who could write a letter of recommendation.

For further information about LENS, please see: http:// lens.unifi.it For the PI group, please see: https://calamailab.ino.cnr.it/