Label-free Optical Voltage Imaging with Diamond: A Path to Sub-micron Electrophysiology
Lecturer: Daniel J. McCloskey - School of Physics - Faculty of Science, The University of Melbourne, Australia
Date: Oct. 24, 2022 11 a.m. - 1 p.m.
Location: Querzoli
Organizer: Sacconi Leonardo

Existing voltage imaging techniques based on arrays of electrodes and fluorescent molecular are forced to
trade-off between spatiotemporal resolution and scale. This can limit their utility for understanding the
network- or tissue-wide consequences of synaptic (or gap-junction) scale activity which is thought to heavily
underlie memory and higher cognitive function and prohibits investigation of mesoscale activity over time
scales commensurate with developmental processes and disease progression. We have recently developed
an optoelectronic technology platform for voltage recording which exploits the charge-coupled fluorescence
of lattice defects in surface-engineered synthetic diamond crystals. These ‘diamond voltage imaging
microscopes’ overcome several existing limitations, enabling wireless and quantitative reporting of local
voltage changes in solution with sub-micron resolution over millimetre scales. I will briefly describe the
principles of this technique from a semiconductor physics perspective before focusing on the future
directions of the technology and some possible use-cases in both neural and cardiac electrophysiology. The
primary aim of this talk is to serve as a starting point for generating ideas and discussion in the biomedical
research community.